منابع مشابه
Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...
متن کاملProtein Expression and Purification
This paper describes methods to produce an isotopically labeled 23 kDa viral membrane protein with purified yield of 20 mg/L of Escherichia coli shake flask culture. This yield is sufficient for NMR structural studies and the protein production methods are simple, straightforward, and rapid and likely applicable to other recombinant membrane proteins expressed in E. coli. The target FHA2 p...
متن کاملProtein Expression and Purification
Article history: Received 5 December 2007 and in revised form 12 March 2008 Available online 25 March 2008
متن کاملProtein Expression and Purification
High-copy plasmids are useful for producing large quantities of plasmid DNA, but are generally inadequate for tightly regulating gene expression. Attempts to suppress expression of genes on high-copy plasmids often results in residual or ‘‘leaky” production of protein. For stringent regulation of gene expression, it is often necessary to excise the gene of interest and subclone it into a low-co...
متن کاملProtein Expression and Purification
Sue-Hong Wang,*,† Tien-Shuh Yang,† Shiang-Ming Lin,† Ming-Shiun Tsai,‡ Shinn-Chih Wu,† and Simon J.T. Mao§,1 *Jen-Te Junior College of Medicine Nursing and Management, Miaoli, Taiwan; †Department of Applied Biology, Pig Research Institute Taiwan, Chunan, Taiwan; ‡Insititute of Molecular Biology, Academic Sinica, Taipei, Taiwan; and §Department of Biological Science and Technology, Research Inst...
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ژورنال
عنوان ژورنال: Klinicka onkologie
سال: 2014
ISSN: 0862-495X,1802-5307
DOI: 10.14735/amko20141s92